Adhesion‐related glycocalyx study: quantitative approach with imaging‐spectrum in the energy filtering transmission electron microscope (EFTEM)

FEBS Letters

Foa, Colette²  Fraterno, Marc³  Ponsonnet, Laurence¹  Vacher, Béatrice¹  Bongrand, Pierre²  Desplat-Jego, Sophie²  Soler, Mireille²  Martin, Jean-Michel¹ 

[1]Ecole Centrale de Lyon, Département de Technologie des Surfaces, URA CNRS 855, 36 Av. Guy de Collongue, 69131 Ecully, France

[2]Unité INSERM 387 `Adhésion Cellulaire', Hôpital Ste Marguerite, 13274 Marseille Cedex 9, France

[3]Service Commun de Microscopie Electronique, Faculté de Médecine, Bd Jean Moulin, 13005 Marseille, France

关键词: Adhesion;    Glycocalyx;    Electron energy loss spectroscopy;    Terbium;    CD43;    TPH-1 cell;   

DOI  :  10.1016/S0014-5793(98)00570-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Large polysaccharide molecules composing the glycocalyx have been shown to prevent cell adhesion. However, this process was not observed microscopically. Terbium labeling, combined with a new quantitative imaging method based on electron energy loss spectroscopy, allowed specific glycocalyx staining with excellent contrast. Image analysis enabled us to compare glycocalyx structure in free membrane areas and contacts between monocytic cells and bound erythrocytes. Apparent glycocalyx thickness, in contact areas, was half of the sum of glycocalyx thicknesses in free areas without label density increase. Ultrastructural immunogold localization of CD43 molecules, a major component of glycocalyx, was also demonstrated to be excluded from contact areas during adhesion. Thus, both approaches strongly suggest that some glycocalyx elements must exit from contact to allow binding of adhesion molecules.

【 授权许可】

Unknown