Promoter elements required for sugar‐repression of the RAmy3D gene for α‐amylase in rice

FEBS Letters

Yamaguchi, Junji²  Umemura, Taka-aki¹  Toyofuku, Kyoko² 

[1] Department of Agriculture, Meijo University, Tenpaku-ku, Nagoya 468-0073, Japan;Bioscience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan

关键词: cis-acting element;    Scutellar epithelium;    G motif;    GATA motif;    Sugar sensing;    Oryza sativa;    GUS;    β-glucuronidase;    LUC;    luciferase;    2;    4-D;    2;    4-dichlorophenoxyacetic acid;    GA;    gibberellin;    ABA;    abscisic acid;   

DOI  :  10.1016/S0014-5793(98)00518-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

There is increasing evidence showing that cereal α-amylase gene expression is controlled not only by the classical hormonal regulation, but also by feed-back sugar repression. We demonstrated by in situ hybridization that the sugar repression of rice α-amylase gene RAmy3D takes place in scutellar epithelium cells of callus-forming rice embryos. We also used a transient expression system to study the cis-acting elements involved in the sugar repression of the RAmy3D promoter activity. Site-directed mutagenesis of the 50-bp nucleotide sequence from −172 to −123 revealed that consensus sequences of G motif (TACGTA) and TATCCA T/C motif (GATA motif as its antisense sequence) are responsible for sugar repression. The promoter sequences required for sugar repression are reported and discussed.

【 授权许可】

Unknown