A novel importin α from rice, a component involved in the process of nuclear protein transport

FEBS Letters

Yamamoto, Naoki²  Matsuki, Rikyu²  Miyao, Mitsue²  Iwasaki, Toshisuke³  Shoji, Kazuhiro¹  Sanmiya, Kazutsuka⁴ 

[1] Central Research Institute of Electric Power Industry, Abiko 270-1194, Japan;National Institute of Agrobiological Resources, 2-1-2 Kannondai, Tsukuba 305-8602, Japan;Department of Biology, Faculty of Science, Niigata University, 8050 Ikarashi 2, Niigata 950-2181, Japan;BioScience Center, Nagoya University, Nagoya 464-8601, Japan

关键词: Importin α;    Nuclear localization signal;    Nuclear protein import;    Rice (Oryza sativa);    GST;    glutathione S-transferase;    IBB;    importin β binding;    NLS;    nuclear localization signal;    NPC;    nuclear pore complex;   

DOI  :  10.1016/S0014-5793(98)00540-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In eukaryotes, nuclear proteins that are transported into nuclei have nuclear localization signals (NLSs), which are recognized by proteins called importin α. We isolated a rice cDNA, #61L, and the corresponding gene that encodes a protein, which shows significant homology to the importin α. Although the encoded protein had only 23–27% amino acid identity to the importin αs from various organisms including plants, the fusion protein with glutathione S-transferase showed a specific binding activity to the NLS of SV40 T-antigen. These results suggest that the rice #61L protein is a novel importin α in plants.

【 授权许可】

Unknown