Saccharomyces cerevisiae mitochondria can synthesise FMN and FAD from externally added riboflavin and export them to the extramitochondrial phase

FEBS Letters

Passarella, Salvatore¹  Pallotta, Maria Luigia¹  Brizio, Carmen²  De Virgilio, Caterina²  Fratianni, Alessandra¹  Barile, Maria² 

[1] Dipartimento di Scienze Animali, Vegetali e dell'Ambiente, via F. De Sanctis, Università del Molise, 86100 Campobasso, Italy;Dipartimento di Biochimica e Biologia Molecolare, Università di Bari, Bari, Italy

关键词: Mitochondrion;    Riboflavin;    Flavin adenine dinucleotide;    Flavin mononucleotide;    Saccharomyces cerevisiae;    FADDS;    flavin adenine dinucleotide detecting system;    RLM;    rat liver mitochondria;    SCM;    Saccharomyces cerevisiae mitochondria;   

DOI  :  10.1016/S0014-5793(98)00544-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Evidence is given that mitochondria isolated from Saccharomyces cerevisiae can take up externally added riboflavin and synthesise from it both flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) probably due to the existence of the mitochondrial riboflavin kinase already reported and the novel mitochondria FAD synthetase. Moreover Saccharomyces cerevisiae mitochondria can export the newly synthesised flavin derivatives to the extramitochondrial phase. This has been proven to take place with 1:1 stoichiometry with riboflavin decrease outside mitochondria, thus showing that flavin traffic occurs across the mitochondrial membranes.

【 授权许可】

Unknown