Cloning and sequencing of ISC1041 from the archaeon Sulfolobus solfataricus MT‐4, a new member of the IS30 family of insertion elements

FEBS Letters

Politi, Laura¹  Ammendola, Sergio¹  Scandurra, Roberto¹ 

[1] Dipartimento di Scienze Biochimiche, Università di Roma `La Sapienza', P.le Aldo Moro 5, 00185 Rome, Italy

关键词: Archaeon;    IS30 family;    Transposase;    DDE motif;    ISC;    insertion sequence of Crenoarcheota;    PPE;    proximal promoter element;    DPE;    distal promoter element;   

DOI  :  10.1016/S0014-5793(98)00530-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A genomic fragment containing the insertion sequence ISC1041 has been cloned by PCR from the archaeon Sulfolobus solfataricus MT-4, an extremophilic microorganism which grows at 87°C. The 1038 bp ISC1041 element contains an imperfect 18 nt repeat and a long open reading frame which encodes a polypeptide of 311 amino acid residues. The translated amino acid sequence shows a significant similarity to IS30-like transposases. Structural analysis indicates that ISC1041 is a novel member of the IS30 family and displays the DDE motif not previously seen in Archaea. This motif is believed to be involved in the integration mechanism of many mobile elements. As this motif is present in several integrases and transposases which, despite the lack of overall protein homologies, share topological homologies to the DDE motif, a common ancestor has been proposed. The finding of an IS30-like transposase in the archaeal kingdom may have relevance for horizontal gene transfer.

【 授权许可】

Unknown