The visual cycle retinol dehydrogenase: possible involvement in the 9‐cis retinoic acid biosynthetic pathway

FEBS Letters

Deutman, A.F¹  Janssen, A.P.M¹  van Vugt, A.H.M¹  Winkens, H.J¹  Driessen, C.A.G.G¹  Janssen, J.J.M¹  Kuhlmann, E.D¹ 

[1] Department of Ophthalmology, University of Nijmegen, Philips van Leydenlaan 15, 6525 EX Nijmegen, Netherlands

关键词: Retinoid metabolism;    Retinol dehydrogenase;    Substrate specificity;    Visual cycle;    Retinoic acid;    RPE;    retinal pigment epithelium;    11-cis-RoDH;    11-cis retinol dehydrogenase;    RT-PCR;    reverse transcriptase polymerase chain reaction;    DoM;    dodecyl-β-1-maltoside;    Sf9;    Spodoptera frugiperda;    9cRDH;    9-cis-retinol dehydrogenase;    Bps;    binding proteins;    CRalBP;    cellular retinal binding protein;    LRAT;    lecithin:retinol acyltransferase;   

DOI  :  10.1016/S0014-5793(98)00473-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 11-cis-retinol dehydrogenase (11-cis-RoDH) gene encodes the short-chain alcohol dehydrogenase responsible for 11-cis-retinol oxidation in the visual cycle. The structure of the murine 11-cis-RoDH gene was used to reinvestigate its transcription pattern. An 11-cis-RoDH gene transcript was detected in several non-ocular tissues. The question regarding the substrate specificity of the enzyme was therefore addressed. Recombinant 11-cis-RoDH was found capable of oxidizing and reducing 9-cis-, 11-cis- and 13-cis-isomers of retinol and retinaldehyde, respectively. Dodecyl-β-1-maltoside used to solubilize the enzyme was found to affect the substrate specificity. This is the first report on a visual cycle enzyme also present in non-retinal ocular and non-ocular tissues. A possible role in addition to its role in the visual cycle is being discussed.

【 授权许可】

Unknown