Constitutive and inducible type 1 ribosome‐inactivating proteins (RIPs) in elderberry (Sambucus nigra L.)

FEBS Letters

Camafeita, Emilio¹  Ferreras, José Miguel²  Méndez, Enrique¹  de Benito, Fernando M²  Girbés, Tomás²  Citores, Lucı́a²  Iglesias, Rosario² 

[1] Centro Nacional de Biotecnologı́a, Consejo Superior de Investigaciones Cientı́ficas (CSIC), Canto Blanco, Madrid, Spain;Departamento de Bioquı́mica y Biologı́a Molecular, Facultad de Ciencias, Universidad de Valladolid, 47005 Valladolid, Spain

关键词: Nigritin;    Ribosome-inactivating protein;    Polynucleotide:adenine glycosidase;    Protein synthesis inhibition;    Elderberry;    Sambucus nigra;    IC50;    concentration of RIP that gives 50% of inhibiton of translation in rabbit reticulocyte lysates;    Nigritins f1 and f2;    basic type 1 RIPs present in elderberry;    rRNA;    ribosomal ribonucleic acid;    SDS-PAGE;    polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate;    RIP(s);    ribosome-inactivating protein(s);   

DOI  :  10.1016/S0014-5793(98)00496-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two novel highly basic type 1 (single chain) ribosome-inactivating proteins (RIPs) with N-glycosidase activity have been found in elderberries (the fruits of Sambucus nigra L.). Mass spectrometry of these RIPs, which we named nigritins f1 and f2, gave M _r values of 24 095 and 23 565, respectively. Both proteins strongly inhibited protein synthesis in rabbit reticulocyte lysates but were inactive against plant ribosomes. Both nigritins have a similar topological activity on pBlueScript SK⁺ DNA as that displayed by dianthin 30. Nigritin f1 is a constitutive RIP since it is present in both green and mature intact elderberries at nearly the same proportion with respect to total fruit protein. By contrast, nigritin f2 is inducible and only appeared in mature intact elderberries. Elderberries also contain two isoforms of a basic nigrin equivalent to the recently found basic nigrin b in elder bark (De Benito et al., FEBS Letters 413 (1997) 85–91). Our results indicate that probably not all plant RIPs exert the same biological function and that this may be determined by the physiological state of the tissue.

【 授权许可】

Unknown