Minimal tRNASer and tRNASec substrates for human seryl‐tRNA synthetase: contribution of tRNA domains to serylation and tertiary structure

FEBS Letters

Heckl, Martha¹  Gross, Hans J¹  Busch, Kristina¹ 

[1] Institut für Biochemie, Bayerische Julius-Maximilians-Universität, Biozentrum, Am Hubland, D-97074 Würzburg, Germany

关键词: tRNASer;    tRNASec;    Seryl-tRNA synthetase;    Microhelix;    RNA structure;    RNA structure probing;   

DOI  :  10.1016/S0014-5793(98)00435-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The recognition process of tRNA^Ser and tRNA^Sec by human seryl-tRNA synthetase (SerRS) was studied using T7 transcripts representing defined regions of human tRNA^Ser or tRNA^Sec and the influence of the tRNA elements on serylation and tertiary structure was elucidated. The anticodon arms of both tRNAs showed no contribution to serylation in contrast to the acceptor stems and the long extra arms. D and T arms were only involved in formation of the L-shaped tRNA structure, not in the recognition process between tRNAs and SerRS. This is the first report of microhelices adapted from human tRNAs being aminoacylated by their homologous synthetase.

【 授权许可】

Unknown