Caveolin‐3 is not an integral component of the dystrophin glycoprotein complex

FEBS Letters

Campbell, Kevin P²  Yamada, Hiroki²  Lisanti, Michael P¹  Venzke, David P²  Crosbie, Rachelle H² 

[1] Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA;Howard Hughes Medical Institute, Department of Physiology and Biophysics, Department of Neurology, University of Iowa College of Medicine, 400 Eckstein Medical Research Building, Iowa City, IA 52242, USA

关键词: Muscle;    Sarcolemma;    Dystrophin-glycoprotein complex;    Caveolin;    Sarcospan;    Cav-3;    caveolin-3;    DGC;    dystrophin-glycoprotein complex;    DG;    dystroglycan;    SG;    sarcoglycan;   

DOI  :  10.1016/S0014-5793(98)00442-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The dystrophin-glycoprotein complex is a multi-subunit protein complex that spans the muscle plasma membrane (sarcolemma) and forms a link between the intracellular cytoskeleton and the extracellular matrix. Caveolin-3, the muscle specific form of caveolin, is also a major structural and regulatory integral membrane protein found at the sarcolemma. Oligomers of caveolin-3 form the structural framework for small membrane pockets known as caveolae. We directly examined whether caveolin-3 is an integral component of the dystrophin-glycoprotein complex by examining four common biochemical and cellular properties of proteins integrally bound to the dystrophin-glycoprotein complex. We found that caveolin-3 de-enriches with partial purification of the dystrophin-glycoprotein complex although a small amount of caveolin-3 is present. Sucrose gradient fractionation and laminin affinity chromatography completely separate this residual caveolin-3 from the core components of the dystrophin-glycoprotein complex. We also show that caveolin-3 expression at the sarcolemma is not reduced in patients with primary mutations in either dystrophin or the sarcoglycans. This data demonstrates that localization of caveolin-3 to the sarcolemma occurs independently of the dystrophin-glycoprotein complex and that caveolin-3 is not an integral component of the dystrophin-glycoprotein complex.

【 授权许可】

Unknown