Xanthine oxidoreductase catalyses the reduction of nitrates and nitrite to nitric oxide under hypoxic conditions

FEBS Letters

Harrison, Roger¹  Stevens, Cliff R²  Benjamin, Nigel³  Blake, David R²  Eisenthal, Robert¹  Millar, Timothy M² 

[1]Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK

[2]Bone and Joint Research Group, Department of Postgraduate Medicine, University of Bath, Claverton Down, Bath, BA2 7AY, UK

[3]Clinical Pharmacology, St. Bartholomew's and the Royal London School of Medicine and Dentistry, Charterhouse Square, London, EC1M 6BQ, UK

关键词: Xanthine oxidoreductase;    Nitric oxide;    Nitrate;    Nitrite;    XOR;    xanthine oxidoreductase;    GTN;    glyceryl trinitrate;    NO;    nitric oxide;   

DOI  :  10.1016/S0014-5793(98)00430-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Xanthine oxidoreductase (XOR) catalyses the reduction of the therapeutic organic nitrate, nitroglycerin (glyceryl trinitrate, GTN), as well as inorganic nitrate and nitrite, to nitric oxide (NO) under hypoxic conditions in the presence of NADH. Generation of nitric oxide is not detectable under normoxic conditions and is inhibited by the molybdenum site-specific inhibitors, oxypurinol and (−)BOF 4272. These enzymic reactions provide a mechanism for generation of NO under hypoxic conditions where nitric oxide synthase does not function, suggesting a vasodilatory role in ischaemia.

【 授权许可】

Unknown