Differential gene expression of a human α2,3‐sialyltransferase in leukaemic cell lines and leucocytes

FEBS Letters

Kelm, Sørge¹  Gassen, Uta¹  Schauer, Roland¹ 

[1] Biochemisches Institut, Christian-Albrechts-Universität Kiel, Otto-Hahn-Platz 9, 24098 Kiel, Germany

关键词: Sialyltransferase;    Differential gene expression;    Leukemic cell line;    AP1;    EMSA;    electrophoretic mobility shift assay;    PMA;    4β-phorbol-12-myristate 13-acetate;    DMSO;    dimethyl sulphoxide;    PMSF;    phenylmethylsulphonyl fluoride;    RT;    reverse transcriptase;    ST3Gal IV;    Galβ1;    4(3)GlcNAc/Galβ1;    3GalNAc-α2;    3-sialyltransferase;   

DOI  :  10.1016/S0014-5793(98)00407-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene expression of the human Galβ1,4(3)GlcNAc/Galβ1,3GalNAcα-2,3-sialyltransferase was investigated in the leukaemic cell lines HL60, K-562, MOLT-4, THP-1 and in blood leucocytes. Five different transcripts were identified. In HL60 and THP-1 cells the expression levels of two of these changed during differentiation. Two potential AP1 binding sites were detected in the promoter regions of the gene. THP-1 cells contain proteins binding with higher affinities to these sequences in the sialyltransferase gene than to the AP1 consensus sequence, whereas nuclear extracts from HL60 cells have the opposite affinity.

【 授权许可】

Unknown