Carbohydrate induced modulation of cell membrane VII. Binding of exogenous lectin increases osmofragility of erythrocytes

FEBS Letters

Pande, Abhay H.¹  Hajela, Namita¹  Hajela, Krishnan¹ 

[1] School of Life Sciences, Devi Ahilya Vishwavidyalaya Vigyan Bhawan, Khandwa Road Campus, Indore-452001 (M.P.), India

关键词: Lectin;    Red cell membrane perturbation;    Osmofragility;    Hemolysis;   

DOI  :  10.1016/S0014-5793(98)00384-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Due to their multivalent binding character, lectins when added exogenously will cross-link membrane surface receptors leading to lateral molecular reorganizations in the plane of the bilayer. This study reports for the first time that agglutination of rabbit erythrocytes by lentil lectin and concanavalin A increases their osmofragility. Increase in osmofragility was detected by measuring the hemolysis of erythrocytes in hypotonic as well as in isotonic solutions. It was also found that agglutination per se does not increase osmofragility but the binding of legume lectin is essential since human Rh⁺ cells agglutinated by a monoclonal antibody do not exhibit hemolysis.

【 授权许可】

Unknown