Xanthine oxidoreductase is asymmetrically localised on the outer surface of human endothelial and epithelial cells in culture

FEBS Letters

Harrison, Roger²  Benboubetra, Mustapha²  Bryant, Richard²  Stevens, Cliff R¹  Tosh, David²  Rouquette, Magali²  Whish, William D²  Page, Susanna²  Blake, David R¹ 

[1] Department of Postgraduate Medicine, University of Bath, Bath BA2 7AY, UK;Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK

关键词: Xanthine oxidoreductase;    Immunolocalization;    Endothelial;    Epithelial;    Human;    XOR;    xanthine oxidoreductase;    FCS;    foetal calf serum;    HUVEC;    human umbilical vein endothelial cell;    DIC;    differential interference contrast;   

DOI  :  10.1016/S0014-5793(98)00385-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Subcellular localisation of xanthine oxidoreductase (XOR) was determined by indirect immunofluorescence using confocal microscopy in human endothelial and epithelial cell lines and in primary cultures of human umbilical vein endothelial cells. XOR was diffusely distributed throughout the cytoplasm but with higher intensity in the perinuclear region. In non-permeabilised cells, XOR was clearly seen to be asymmetrically located on the outer surfaces, showing, in many cases, a higher intensity on those faces apposed by closely neighbouring cells. Such specific distribution suggests a functional role for the enzyme in cell-cell interactions, possibly involving signalling via reactive oxygen species

【 授权许可】

Unknown