Non‐balanced mix of mitochondrial DNA in cloned cattle produced by cytoplast‐blastomere fusion

FEBS Letters

Brem, Gottfried²  Müller, Mathias²  Wolf, Eckhard³  Steinborn, Ralf²  Zakhartchenko, Valeri¹ 

[1] Bavarian Research Centre of Reproductive Biology, Hackerstr. 27, D-85764 Oberschleißheim, Germany;Department of Animal Biotechnology at IFA, A-3430 Tulln, Austria;Department of Molecular Animal Breeding and Genetics, Ludwig-Maximilian University, Veterinärstr. 13, D-80539 Munich, Germany

关键词: Cloning;    Cattle;    mtDNA;    Control region;    Allele-specific quantitation;    Allele-specific TaqMan polymerase chain reaction;    DB;    donor blastomere;    RC;    recipient cytoplast;    CR;    control region;    AS-TaqMan PCR;    allele-specific TaqMan polymerase chain reaction;    NTC;    no template control;    NAC;    no amplification control;    Ta;    annealing temperature;   

DOI  :  10.1016/S0014-5793(98)00351-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have investigated the transmission of parental mitochondrial DNA (mtDNA) in three clones of born cattle obtained by intraspecific cytoplast-blastomere fusion. Using allele-specific TaqMan PCR a low level transmission of blastomere mtDNA (DB mtDNA) into the cloned offspring was detected, thereby generating a heteroplasmic population of mtDNA. The amount of DB mtDNA was 13% and 18% in two animals of a clone which derived from a 24-cell morula and 0.6% and 0.4% in two calves of clonal origin derived from a 92-cell morula. These values are in accordance with the tendency expected for neutral mtDNA segregation that the fewer cell divisions that have occurred in the donor embryo, the higher the amount of DB mtDNA. We also found a strong decrease of DB mtDNA which was about three orders of magnitude in the third clone derived from a 52-cell morula stage.

【 授权许可】

Unknown