Composition of parental mitochondrial DNA in cloned bovine embryos

FEBS Letters

Brem, Gottfried³  Müller, Mathias³  Wolf, Eckhard⁴  Jelyazkov, Jivko³  Klein, Dieter²  Steinborn, Ralf³  Zakhartchenko, Valeri¹ 

[1]Bavarian Research Centre of Reproductive Biology, Hackerstr. 27, D-85764 Oberschleißheim, Germany

[2]Institute of Virology, University of Veterinary Sciences, Veterinärplatz 1, A-1210 Vienna, Austria

[3]Department of Animal Biotechnology at IFA, A-3430 Tulln, Austria

[4]Department of Molecular Animal Breeding and Genetics, Ludwig-Maximilian University, Veterinärstr. 13, D-80539 Munich, Germany

关键词: Cloning;    Bovine;    Mitochondrial DNA;    Fusion;    Allele-specific quantitation;    TaqMan™ polymerase chain reaction;    DB;    donor blastomere;    RC;    recipient cytoplast;    CR;    control region;    AS-PCR;    allele-specific PCR;    NTC;    no template control;    NAC;    no amplification control;    T a;    annealing temperature;    PIRA;    primer introduced restriction analysis;   

DOI  :  10.1016/S0014-5793(98)00350-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have investigated parental mitochondrial DNA (mtDNA) in cloned bovine embryos obtained by intraspecific cytoplast-blastomere fusion. Analysis of two-cell to blastocyst stage embryos revealed that in contrast to the exclusion of paternal (sperm) mtDNA during sexual inheritance in the cytoplast-blastomere fusion complexes, there was mixing and co-existence of parental mtDNA. The mixing of mtDNA was non-balanced with the minority deriving from the blastomere. The constant content of mtDNA during embryogenesis until the blastocyst stage suggesting an absence of mtDNA replication was shown for conventional `in vitro fertilised' (IVF) embryos and for cloned embryos. The ratio of parental mtDNA was in accordance with the estimated quantitative participation of mtDNA from the fusion partners.

【 授权许可】

Unknown