FEBS Letters | |
Composition of parental mitochondrial DNA in cloned bovine embryos | |
Brem, Gottfried3 Müller, Mathias3 Wolf, Eckhard4 Jelyazkov, Jivko3 Klein, Dieter2 Steinborn, Ralf3 Zakhartchenko, Valeri1 | |
[1] Bavarian Research Centre of Reproductive Biology, Hackerstr. 27, D-85764 Oberschleißheim, Germany;Institute of Virology, University of Veterinary Sciences, Veterinärplatz 1, A-1210 Vienna, Austria;Department of Animal Biotechnology at IFA, A-3430 Tulln, Austria;Department of Molecular Animal Breeding and Genetics, Ludwig-Maximilian University, Veterinärstr. 13, D-80539 Munich, Germany | |
关键词: Cloning; Bovine; Mitochondrial DNA; Fusion; Allele-specific quantitation; TaqMan™ polymerase chain reaction; DB; donor blastomere; RC; recipient cytoplast; CR; control region; AS-PCR; allele-specific PCR; NTC; no template control; NAC; no amplification control; T a; annealing temperature; PIRA; primer introduced restriction analysis; | |
DOI : 10.1016/S0014-5793(98)00350-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We have investigated parental mitochondrial DNA (mtDNA) in cloned bovine embryos obtained by intraspecific cytoplast-blastomere fusion. Analysis of two-cell to blastocyst stage embryos revealed that in contrast to the exclusion of paternal (sperm) mtDNA during sexual inheritance in the cytoplast-blastomere fusion complexes, there was mixing and co-existence of parental mtDNA. The mixing of mtDNA was non-balanced with the minority deriving from the blastomere. The constant content of mtDNA during embryogenesis until the blastocyst stage suggesting an absence of mtDNA replication was shown for conventional `in vitro fertilised' (IVF) embryos and for cloned embryos. The ratio of parental mtDNA was in accordance with the estimated quantitative participation of mtDNA from the fusion partners.
【 授权许可】
Unknown
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