Examination of temperature‐induced ‘gel‐sol’ transformation of α‐actinin/cross‐linked actin networks by static light scattering

FEBS Letters

Goldmann, Wolfgang H.¹  Guttenberg, Zeno¹ 

[1] Surgery Research Laboratories, Massachusetts General Hospital, Harvard Medical School, Building 149, 13th Street, Charlestown, MA 02129, USA

关键词: Actin;    α-Actinin;    Binding kinetics;    Light scattering;    T g;    temperature-dependent gel-sol transition point;    ΔI;    change in light scatter intensity;    K;    equilibrium constant;    UV;    ultraviolet light;    p α;    portion of bound α-actinin to actin;    ΔH;    heat of association;    K 0;    proportionality constant;    R;    gas constant;    T;    absolute temperature (Kelvin);    (I)T;    temperature-dependent light scatter intensity;    I=I s/I o;    light scatter intensity equals the ratio of incident/reflected light;    [α];    α-actinin concentration;    [A];    F-actin concentration;    [C];    α-actinin/F-actin complex;    p α(T);    temperature-dependent portion of bound α-actinin to actin;    J(t);    time-dependent creep;    r Aα;    ratio of F-actin to α-actinin;    r AG;    ratio of F-actin to gelsolin;   

DOI  :  10.1016/S0014-5793(98)00353-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We studied the gel-sol transformation of F-actin/α-actinin solutions. Cross-linking of actin filaments by α-actinin shows a temperature-dependent increase in light scatter signal, (I)T. Higher F-actin/α-actinin molar ratios, r _Aα as well as increases in F-actin concentration, [A], and reduction of actin filament lengths, r _AG, augment the maximal light intensity, I and shift the gel-sol transition point, T _g to higher temperatures. This behavior is interpreted in terms of the model developed by Tempel, M., Isenberg, G. and Sackmann, E. (1996) (Physical Review E 54, 1802–1810) based on the percolation theory. Using the temperature-dependent binding model of this theory allows instant prediction of the equilibrium constant, K for F-actin/α-actinin solutions at temperatures T<T _g.

【 授权许可】

Unknown