期刊论文详细信息
FEBS Letters
Increased cellular resistance to oxidative stress by expression of cyanobacterium catalase‐peroxidase in animal cells
Takeda, Toru1  Ohta, Yuriko2  Nishikimi, Morimitsu2  Ishikawa, Takahiro2  Shigeoka, Shigeru1 
[1] Department of Food and Nutrition, Faculty of Agriculture, Kinki University, Nara 631-8505, Japan;Department of Biochemistry, Wakayama Medical College, Wakayama 640-8155, Japan
关键词: Catalase-peroxidase;    Oxidative stress;    Expression;    Synechococcus PCC 7942;    104C1 cell;    CPX;    catalase-peroxidase;    S.7942;    Synechococcus PCC 7942;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    PBS;    phosphate-buffered saline;    MTT;    3-(4;    5-dimethyl-2-thiazolyl)-2;    5-diphenyltetrazolium bromide;   
DOI  :  10.1016/S0014-5793(98)00347-0
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

To exploit prokaryotic antioxidant enzymes for protection of animal cells from oxidative damage, we expressed catalase-peroxidase of cyanobacterium Synechococcus PCC 7942 in 104C1 cells. The gene for this enzyme was inserted into the mammalian expression vector pRc/CMV. The stable transfectants obtained had higher specific activities of catalase and as a result became more resistant to H2O2 or paraquat than the parental cells. Subcellular fractionation and immunoblot analysis revealed that the expressed catalase-peroxidase was confined to the cytosol; this localization may be the basis for the effective protection of the transfectants from the oxidative cell damage.

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