Increased cellular resistance to oxidative stress by expression of cyanobacterium catalase‐peroxidase in animal cells

FEBS Letters

Takeda, Toru¹  Ohta, Yuriko²  Nishikimi, Morimitsu²  Ishikawa, Takahiro²  Shigeoka, Shigeru¹ 

[1] Department of Food and Nutrition, Faculty of Agriculture, Kinki University, Nara 631-8505, Japan;Department of Biochemistry, Wakayama Medical College, Wakayama 640-8155, Japan

关键词: Catalase-peroxidase;    Oxidative stress;    Expression;    Synechococcus PCC 7942;    104C1 cell;    CPX;    catalase-peroxidase;    S.7942;    Synechococcus PCC 7942;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    PBS;    phosphate-buffered saline;    MTT;    3-(4;    5-dimethyl-2-thiazolyl)-2;    5-diphenyltetrazolium bromide;   

DOI  :  10.1016/S0014-5793(98)00347-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To exploit prokaryotic antioxidant enzymes for protection of animal cells from oxidative damage, we expressed catalase-peroxidase of cyanobacterium Synechococcus PCC 7942 in 104C1 cells. The gene for this enzyme was inserted into the mammalian expression vector pRc/CMV. The stable transfectants obtained had higher specific activities of catalase and as a result became more resistant to H₂O₂ or paraquat than the parental cells. Subcellular fractionation and immunoblot analysis revealed that the expressed catalase-peroxidase was confined to the cytosol; this localization may be the basis for the effective protection of the transfectants from the oxidative cell damage.

【 授权许可】

Unknown