Identification and location on syndecan‐1 core protein of the epitopes of B‐B2 and B‐B4 monoclonal antibodies

FEBS Letters

Wijdenes, John²  Morard, Florence²  Vita, Natalio¹  Dore, Jean-Michel² 

[1] Sanofi, Labège, France;Diaclone, 1 bvd. A. Fleming, P.O. Box 1985, F-25020 Besançon Cedex, France

关键词: B-B2 monoclonal antibody;    B-B4 monoclonal antibody;    Syndecan-1;    Epitope characterization;    Peptide phage display;   

DOI  :  10.1016/S0014-5793(98)00310-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a phage display peptide library, we characterized the epitope of two monoclonal antibodies reacting with syndecan-1: B-B2 and B-B4. The identified epitopes QDIT, for B-B2, and LPEV, for B-B4, were found to align with residues 36–39 and 90–93 of the mature protein, respectively. In contrast to B-B4, the B-B2 epitope is close to a potential glycosaminoglycan attachment site. Since syndecan-1 is heavily glycosylated and post-translational modifications are cell type specific, these results might explain the differences observed in the reactivity pattern of B-B2 and B-B4 and suggest that these monoclonal antibodies are useful probes to study cell surface exposed syndecan-1.

【 授权许可】

Unknown