FEBS Letters | |
Maize polyamine oxidase: primary structure from protein and cDNA sequencing | |
Rea, Giuseppina2  Schininà, M.Eugenia1  Tavladoraki, Paraskevi2  Mariottini, Paolo2  Angelini, Riccardo2  Federico, Rodolfo2  Manera, Francesco1  Di Agostino, Silvia2  Cecconi, Francesco2  | |
[1] Dipartimento di Scienze Biochimiche `A. Rossi Fanelli' and Centro di Biologia Molecolare del Consiglio Nazionale delle Ricerche, Università `La Sapienza', Piazzale Aldo Moro 5, 00185 Rome, Italy;Dipartimento di Biologia, Università `Roma Tre', Viale Guglielmo Marconi 446, 00146 Rome, Italy | |
关键词: Flavin oxidase; Hydrogen peroxide; Polyamine oxidase; Primary structure; Zea mays L.; dNTP; deoxynucleoside triphosphate; MAO; monoamine oxidase; MPAO; maize polyamine oxidase; RACE; rapid amplification of cDNA ends; RT-PCR; reverse transcription-polymerase chain reaction; SDS-PAGE; sodium dodecylsulfate polyacrylamide gel electrophoresis; UTR; untranslated region; | |
DOI : 10.1016/S0014-5793(98)00311-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The first complete amino acid sequence of a flavin-containing polyamine oxidase was solved by a combined approach of nucleotide and peptide sequence analysis. A cDNA of 1737 bp, isolated from maize seedlings by reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends strategies, was cloned and its sequence determined. This cDNA contains information for a polypeptide chain of 500 amino acids. Its amino-terminal sequence shows the typical features of secretion signal peptides. The primary structure of the mature protein was independently confirmed by extensive amino acid sequencing. Structural relationships with flavin-containing monoamine oxidases are also discussed.
【 授权许可】
Unknown
【 预 览 】
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RO201912020305804ZK.pdf | 349KB | download |