Human retinoblastoma protein (Rb) is phosphorylated by cdc2 kinase and MAP kinase in Xenopus maturing oocytes

FEBS Letters

Taieb, Frédéric¹  Jessus, Catherine¹  Karaiskou, Anthi¹  Rime, Hélène¹ 

[1] Laboratoire de Physiologie de la Reproduction, INRA/URA-CNRS 1449, Université Pierre et Marie Curie, Boı̂te 13, 4 place Jussieu, 75252 Paris Cedex 05, France

关键词: Xenopus;    Meiotic maturation;    Rb kinase;    cdk;    Cyclin;    Mitogen-activated protein kinase;    GST;    glutathione S-transferase;    GVBD;    germinal vesicle breakdown;    MAP kinase;    mitogen-activated protein kinase;    MBP;    myelin basic protein;    Rb;    retinoblastoma gene product;   

DOI  :  10.1016/S0014-5793(98)00291-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Xenopus oocyte meiotic maturation combines features of G0/G1 and G2/M transitions of the cell cycle. To study the in ovo Rb kinase activity, we have microinjected human Rb into oocytes. Microinjected human Rb localizes into the nucleus, is hypophosphorylated in prophase oocytes, becomes hyperphosphorylated during meiotic maturation and is dephosphorylated as the cell reenters interphase. Inactivation or overexpression of the cyclin D-cdk4/6 complex in an oocyte extract does not affect the Rb kinase activity. This kinase activity could be attributed to both cdc2-cyclin B and MAP kinase, opening new perspectives of investigation in somatic cells.

【 授权许可】

Unknown