Phenobarbital induces cytochrome P4501A2 hnRNA, mRNA and protein in the liver of C57BL/6J wild type and aryl hydrocarbon receptor knock‐out mice

FEBS Letters

Marc, Nathalie²  Guillouzo, André²  Pineau, Thierry¹  Wein, Sharon¹  Fautrel, Alain²  Corcos, Laurent² 

[1]Laboratoire de Pharmacologie Toxicologie, INRA, 180, Chemin de Tournefeuille, P.O. Box 3, 31931 Toulouse, France

[2]INSERM U456, Faculté de Pharmacie, 2, Avenue Léon Bernard, 35043 Rennes Cedex, France

关键词: Phenobarbital;    Cytochrome P450;    Aryl hydrocarbon receptor;    Knock-out mouse;    Liver;    AHR;    aryl hydrocarbon receptor;    AHR−/−;    knocked out AHR gene;    Ah;    aryl hydrocarbon;    CYP;    cytochrome P450;    ARNT;    aryl hydrocarbon receptor nuclear translocator;    PAS;    Per-ARNT-Sim;    XRE;    xenobiotic response element;    DRE;    drug response element;    3-MC;    3-methylcholanthrene;    PB;    phenobarbital;    PAH;    polycyclic aromatic hydrocarbon;    wt;    wild type;    GSTYα;    glutathione S-transferase alpha class;    EDTA;    ethylene diamine tetraacetic acid;    TBE;    Tris-borate-EDTA;   

DOI  :  10.1016/S0014-5793(98)00246-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The aryl hydrocarbon receptor mediates the transcriptional response to a variety of hydrocarbons of members of the aryl hydrocarbon gene battery. Phenobarbital does not bind the aryl hydrocarbon receptor with high affinity but induces, in liver cells, expression of cytochrome P4501A. Using both wild type and aryl hydrocarbon receptor knock out C57BL/6J mice, we demonstrate that phenobarbital induced hnRNA, mRNA and protein for the cytochrome P-4501A2 gene in the presence or absence of the aryl hydrocarbon receptor. Using the DNA binding site for the aryl hydrocarbon receptor as a probe, gel retardation analyses showed that phenobarbital treatment induced protein binding, regardless of the presence of the aryl hydrocarbon receptor.

【 授权许可】

Unknown