Contribution of NO synthases to neutrophil infiltration in the gastric mucosal lesions in rats with water immersion restraint stress

FEBS Letters

Ohta, Yoshiji¹  Nishida, Keiji¹  Ishiguro, Isao¹ 

[1] Department of Biochemistry, School of Medicine, Fujita Health University, Toyoake, Aichi 470-1192, Japan

关键词: Water immersion restraint stress;    Gastric mucosal injury;    Constitutive NO synthase;    Inducible NO synthase;    Myeloperoxidase;    Neutrophil infiltration;    cNOS;    constitutive nitric oxide synthase;    iNOS;    inducible nitric oxide synthase;    MPO;    myeloperoxidase;    D- or L-NMMA;    N G-monomethyl d- or l-arginine;    respectively;    NO;    nitric oxide;    WIR;    water immersion restraint;   

DOI  :  10.1016/S0014-5793(98)00237-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A decrease in constitutive NO synthase (cNOS) activity and an increase in inducible NO synthase (iNOS) activity occurred with an increase in myeloperoxidase (MPO) activity, an index of neutrophil infiltration, in the gastric mucosa of rats with water immersion restraint (WIR) stress. This increase in gastric mucosal MPO activity was enhanced by pretreatment with N ^G-monomethyl l-arginine, a non-selective NOS inhibitor, but was prevented with maintenance of gastric mucosal cNOS activity by pretreatment with aminoguanidine, a selective iNOS inhibitor. The MPO activity was negatively correlated with the cNOS activity in all WIR-stressed rats used (r=−0.723). These results suggest that a decrease in cNOS activity could contribute to an increase in neutrophil infiltration in the gastric mucosa of WIR-stressed rats.

【 授权许可】

Unknown