Cloning, sequencing and expression of the genes encoding the sodium translocating N 5‐methyltetrahydromethanopterin:coenzyme M methyltransferase of the methylotrophic archaeon Methanosarcina mazei Gö1

FEBS Letters

Lienard, Tanja¹  Gottschalk, Gerhard¹ 

[1] Institut für Mikrobiologie und Genetik der Universität, Grisebachstr. 8, D-37077 Göttingen, Germany

关键词: N 5-Methyltetrahydromethanopterin:coenzyme M methyltransferase;    Sodium ion translocation;    Corrinoid;    Methanogenic archaeon;   

DOI  :  10.1016/S0014-5793(98)00229-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The N ⁵-methyltetrahydromethanopterin:coenzyme M methyltransferase of Methanosarcina mazei Gö1 is a membrane-associated, corrinoid-containing protein that uses a transmethylation reaction to drive an energy-conserving sodium ion pump. The eight open reading frames encoding the eight different subunits of the methyltransferase were identified and sequenced. All of these subunits are shown to be heterologously expressed in minicells of the Escherichia coli mutant DK6. Sequence comparisons with the methyltransferases of thermophilic and hypothermophilic methanogenic archaea are presented. The participation of the gene product of mtrD in sodium ion translocation as well as a consensus sequence of a corrinoid binding motif in MtrA are discussed.

【 授权许可】

Unknown