Identification of an ABC transporter gene that exhibits mRNA level overexpression in fluoroquinolone‐resistant Mycobacterium smegmatis

FEBS Letters

Banerjee, Sanjiban K¹  Bhatt, Kamlesh¹  Misra, Parimal¹  Chakraborti, Pradip K¹  Mande, Shekhar C¹ 

[1] Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India

关键词: ABC transporter;    Active efflux;    Phosphate specific transport;    Drug resistance;    Fluoroquinolone;    Mycobacterium;   

DOI  :  10.1016/S0014-5793(98)00214-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We describe here the PCR amplification of a DNA fragment (mtp1) from Mycobacterium smegmatis using primers derived from consensus sequences of the ABC family of transporters. The fragment encodes amino acid sequences that exhibited significant homology with different ABC transporters. Amino acid sequence alignment of the full length gene with other transporters identified the ABC protein as the B-subunit of the phosphate specific transporter. Strikingly, a M. smegmatis colony which exhibited a high level of ciprofloxacin resistance showed mRNA level overexpression of mtp1. Thus this is the first report in any prokaryote indicating differential expression of an ABC transporter in a fluoroquinolone resistant colony.

【 授权许可】

Unknown