A somatostatin receptor 1 selective ligand inhibits Ca2+ currents in rat insulinoma 1046‐38 cells

FEBS Letters

Glassmeier, Günter²  Meyerhof, Wolfgang¹  Roosterman, Dirk¹  Baumeister, Hans¹  Scherübl, Hans² 

[1] Abteilung Molekulare Genetik, Deutsches Institut für Ernährungsforschung, Arthur-Scheunert-Allee 114–116, D-14558 Potsdam-Rehbrücke, Germany;Abteilung für Innere Medizin/Gastroenterologie, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Hindenburgdamm 30, D-12200 Berlin, Germany

关键词: Neuropeptide;    Ca2+ channel;    Adenylyl cyclase;    Insulinoma;    Receptor;    Somatostatin;    SST;    somatostatin;    sst;    somatostatin receptor subtype;   

DOI  :  10.1016/S0014-5793(98)00221-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rat insulinoma 1046-38 cells represent a model system to study β-cell function. The mRNAs for sst1 and sst2, two of the five somatostatin receptors, were detected by reverse transcription polymerase chain reaction amplification in these cells. Displacement binding analysis suggested that sst1 represents the major somatostatin receptor subtype. The sst1 selective compound CH-275 did not inhibit adenylyl cyclases while compounds that activated sst2 did. In contrast, CH-275 caused a marked inhibition of voltage-operated Ca²⁺ channels while the sst2 specific analog octreotide elicited a less pronounced effect suggesting that in rat insulinoma 1046-38 cells sst1 preferably mediates the inhibition of Ca²⁺ channels.

【 授权许可】

Unknown