Altered expression of α‐actin, smooth muscle myosin heavy chain‐1 and calponin in cultured smooth muscle cells by oxidized low density lipoproteins

FEBS Letters

Shimamura, Kyoko²  Sugiyama, Tomoyasu²  Matsuda, Yuzuru²  Greci, Lucedio¹  Damiani, Elisabetta¹ 

[1]Dipartimento di Scienze dei Materiali e della Terra, Via Brecce Bianche, Università, I-60131 Ancona, Italy

[2]Vessel Research Laboratory Co. Ltd, 3-6-6 Asahi-machi, Machida-shi, Tokyo 194, Japan

关键词: Oxidized low density lipoprotein;    Smooth muscle cell;    α-Actin;    Smooth muscle myosin heavy chain-1;    Calponin;    Atherosclerosis;   

DOI  :  10.1016/S0014-5793(98)00212-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The expression of the contractile proteins, α-actin, smooth muscle myosin heavy chain-1 (SM1) and calponin present in smooth muscle cells (SMC) in the presence of oxidized low density lipoproteins (oxLDL) was investigated in two different cell cultures: the mouse smooth muscle cell line SVSC and rat smooth muscle cells (RSMC). Exposure of the cells to 187 μg protein/ml oxLDL for 24 h reduced the expression of all three contractile proteins in both cell cultures when compared to cells incubated in the presence of native LDL. This investigation of the response of SMC contractile proteins to oxLDL may provide further insights into the mechanisms by which oxidatively modified LDL is atherogenic and suggests that oxLDL may contribute to the regulation of the expression of the genes responsible for the synthesis of smooth muscle cell contractile proteins.

【 授权许可】

Unknown