Elderberry (Sambucus nigra) contains truncated Neu5Ac(α‐2,6)Gal/GalNAc‐binding type 2 ribosome‐inactivating proteins

FEBS Letters

Barre, Annick¹  van Leuven, Fred²  Peumans, Willy J³  Rouge, Pierre¹  van Damme, Els J.M³  Roy, Soma³ 

[1] Institut de Pharmacologie et Biologie Structurale, Université Paul Sabatier, 35 Chemin des Maraı̂chers, UPR CNRS 9062, 31062 Toulouse Cedex, France;Center for Human Genetics, Katholieke Universiteit Leuven, Herestraat 49, 3001 Leuven, Belgium;Laboratory for Phytopathology and Plant Protection, Katholieke Universiteit Leuven, Willem de Croylaan 42, 3001 Leuven, Belgium

关键词: Polynucleotide:adenosine glycosidase;    Ribosome-inactivating protein;    Sambucus;    PB;    phosphate buffer;    RIP;    ribosome-inactivating protein;    SNA;    Sambucus nigra agglutinin;    TrSNAI;    truncated SNAI;    TrSNAIf;    truncated SNAI from fruits;   

DOI  :  10.1016/S0014-5793(98)00193-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Analysis of affinity-purified preparations of the fetuin-binding proteins from elderberry bark and fruits revealed besides the previously reported Neu5Ac(α-2,6)Gal/GalNAc-specific type 2 ribosome-inactivating proteins (RIP) the occurrence of single chain proteins of 22 kDa, which according to their N-terminal amino acid sequence correspond to the second part of the B chain of the respective type 2 RIP. Both proteins are very similar except that the polypeptides of the fruit lectin are 10 amino acid residues longer than these from the bark lectin. Our findings not only demonstrate the occurrence of carbohydrate-binding fragments of type 2 RIP but also provide further evidence that type 2 RIP genes give rise to complex mixtures of type 2 RIP/lectins in elderberry.

【 授权许可】

Unknown