The olfactory adenylyl cyclase type 3 is expressed in male germ cells

FEBS Letters

Hanoune, Jacques¹  Jégou, Bernard³  Defer, Nicole¹  Poyard, Madeleine¹  Marinx, Olivier²  Lienard, Marie Odile³ 

[1] U-99 INSERM, Hopital Henri Mondor, F-94010 Créteil, France;Sanofi Recherche, Labège Innopole, 31676 Labège cedex, France;GERM-INSERM U 435, Université de Rennes I, Campus de Beaulieu, 35042 Rennes cedex, France

关键词: Adenylyl cyclase;    Signal transduction;    Immunohistochemistry;    Germ cell;   

DOI  :  10.1016/S0014-5793(98)00178-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Elements of the olfactory pathway, such as receptors, receptor-desensitization machinery, and cyclic nucleotide-gated channels, are expressed in male germ cells. Here we report the expression, in rat testis, of both adenylyl cyclase type 3 (AC3) and the olfactory G protein subunit, G_αolf. Both are expressed in the same sub-population of germ cells, pachytene spermatocytes to spermatids, and in residual bodies. Neither AC3 nor G_αolf was found in Sertoli or in peritubular cells, as shown by Western blotting and immunocytochemical analyses. It thus appears that male germ cells contain all the elements of the signaling cascade present in olfactory cells.

【 授权许可】

Unknown