FEBS Letters | |
Involvement of protein‐tyrosine phosphorylation and dephosphorylation in sperm‐induced Xenopus egg activation | |
Sato, Ken-ichi1  Tamaki, Ikuo2  Aoto, Mamoru2  Iwasaki, Tetsushi2  Tokmakov, Alexander A2  Fukami, Yasuo1  | |
[1] Laboratory of Molecular Biology, Biosignal Research Center, Kobe University, Nada, Kobe 657, Japan;The Graduate School of Science and Technology, Kobe University, Nada, Kobe 657, Japan | |
关键词: Protein tyrosine phosphorylation/dephosphorylation; Xenopus laevis fertilization; Egg activation; Genistein; Xyk; Xenopus tyrosine kinase; Na3VO4; sodium orthovanadate; DMSO; dimethylsulfoxide; DB; DeBoer's solution; SDS-PAGE; SDS-polyacrylamide gel electrophoresis; MAP kinase; mitogen-activated protein kinase; | |
DOI : 10.1016/S0014-5793(98)00123-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We have analyzed tyrosine-phosphorylated proteins in Xenopus laevis eggs before and after fertilization by immunoblotting with anti-phosphotyrosine antibody. A number of egg proteins with different subcellular distribution became tyrosine-phosphorylated or dephosphorylated within 30 min after insemination. Tyrosine kinase-specific inhibitors genistein and herbimycin A were found to inhibit sperm-induced egg activation judged by the egg cortical contraction. Surprisingly, sodium orthovanadate, a tyrosine phosphatase inhibitor, also inhibited the egg activation. Moreover, we found that fertilization-dependent tyrosine dephosphorylation of 42-kDa mitogen-activated protein kinase was inhibited in genistein-treated eggs. These results suggest that both protein-tyrosine phosphorylation and dephosphorylation pathways play an important role in the sperm-induced Xenopus egg activation.
【 授权许可】
Unknown
【 预 览 】
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