Identification of a lipoxygenase inhibitor in A431 cells as a phospholipid hydroperoxide glutathione peroxidase

FEBS Letters

Lu, Hsieng S²  Chang, Wen-Chang¹  Chen, Ching-Jiunn¹  Huang, Hei-Sheng¹ 

[1] Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, ROC;Amgen Inc., Amgen Center, Thousand Oaks, CA 91320, USA

关键词: Lipoxygenase inhibitor;    Microsequencing;    PHGPx;    A431 cell;   

DOI  :  10.1016/S0014-5793(98)00130-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An endogenous lipoxygenase inhibitor, purified from the cytosol of human epidermoid carcinoma A431 cells, was analyzed by N-terminal microsequencing and mass spectrometric analysis. The inhibitor was purified by SDS-PAGE, then subjected to in-gel CNBr cleavage and trypsin digestion. The N-terminal sequence data obtained from a 6–8 kDa band of in-gel CNBr cleavage and the three isolated peptides of in-gel trypsin digestion, and the C-terminal peptide sequence from matrix-assisted laser desorption ionization mass spectrometry matched the sequence of human phospholipid hydroperoxide glutathione peroxidase. The purified inhibitor exhibited peroxidase activity using phosphatidylcholine hydroperoxides as the substrate. We therefore concluded that the lipoxygenase inhibitor present in A431 cells was a phospholipid hydroperoxide glutathione peroxidase.

【 授权许可】

Unknown