Presence and localization of oscillin in human spermatozoa in relation to the integrity of the sperm membrane

FEBS Letters

Montag, M²  Parrington, J³  Swann, K³  van der Ven, H²  Lai, F.A¹ 

[1] MRC National Institute for Medical Research, Mill Hill, London, UK;Department of Endocrinology and Reproductive Medicine, University of Bonn, 53105 Bonn, Germany;Department of Anatomy and Developmental Biology, University College, London, UK

关键词: Oocyte activation;    Hypo-osmotic swelling test;    Intracytoplasmic sperm injection;    Oscillin;    Sperm cytosol;    Human;   

DOI  :  10.1016/S0014-5793(98)00107-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the presence and localization of oscillin in human spermatozoa in relation to the integrity of the sperm membrane, which was assessed by the hypo-osmotic swelling (HOS) test. We found no gross differences in the presence of oscillin in semen samples from men who presented with 70%, 40%, 25% or 2% of membrane-intact spermatozoa. By immunofluorescence, membrane-intact (HOS-positive) spermatozoa showed staining of a single band at the equatorial region, whereas over 80% of HOS-negative spermatozoa consistently showed a diffuse distribution of oscillin over the sperm head. However, some individuals presented with up to 50% of HOS-positive spermatozoa showing an aberrant localization of oscillin. We found a significant correlation rate (r=0.70, P<0.05) between the percentage of HOS-positive spermatozoa with an equatorial oscillin localization and the fertilization rates achieved after intracytoplasmic sperm injection. These data suggest that the localization of oscillin in human spermatozoa might have an impact on egg activation and fertilization rates.

【 授权许可】

Unknown