Colon carbonic anhydrase 1: transactivation of gene expression by the homeodomain protein Cdx2

FEBS Letters

Edwards, Y.H¹  Morrison, K¹  Drummond, F.-J¹  Sowden, J¹ 

[1] MRC Human Biochemical Genetics Unit, University College London, Wolfson House, 4, Stephenson Way, London NW1 2HE, UK

关键词: Intestinal gene regulation;    Carbonic anhydrase;    Cdx2;    Caudal-type homeobox protein;    Colon promoter;   

DOI  :  10.1016/S0014-5793(98)00103-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The homeodomain protein, Cdx2, has been implicated in the transcriptional regulation of genes expressed in the small intestine. In vitro studies of the carbonic anhydrase 1 (CA1) colon promoter implied that Cdx2 may also play a role in the regulation of colon-specific gene expression. The current work follows up this proposal by examining the ability of Cdx2 to transactivate gene expression in cultured cells mediated by CA1 promoter sequences. The results show that Cdx2 exerts a positive regulatory effect by binding to a motif 87 bp upstream of the CA1 TATA box; this motif appears to act as an enhancer since gene activation is independent of its orientation.

【 授权许可】

Unknown