FEBS Letters | |
Sodium channel protein expression enhances the invasiveness of rat and human prostate cancer cells | |
Djamgoz, Mustafa B.A2  Ke, Youqiang1  Foster, Christopher S1  Fraser, Scott P2  Smith, Paul1  Rhodes, Nicholas P3  Shortland, Adam P3  | |
[1] Department of Cellular and Molecular Pathology, University of Liverpool, Liverpool L69 3GA, UK;Department of Biology, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK;Department of Clinical Engineering, University of Liverpool, Liverpool L69 3GA, UK | |
关键词: Prostatic carcinoma; Flow cytometry; Na+ channel; Metastasis; Tissue culture; | |
DOI : 10.1016/S0014-5793(98)00050-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Expression of Na+ channel protein was analysed in established cell lines of rat and human prostatic carcinoma origin by flow cytometry using a fluorescein-labelled polyclonal antibody. In many cell lines examined, the obtained frequency distribution profiles were bimodal and identified a subpopulation of cells which expressed high levels of Na+ channel protein. A significant positive correlation was demonstrated between the proportion of channel-expressing cells and the functional ability of individual cell lines to invade a basement membrane matrix in vitro. In addition, two transfectant cell lines containing rat prostate cancer genomic DNA were found to express significantly elevated levels of Na+ channel protein when compared with the original benign recipient cell line. Enhanced Na+ channel expression by two metastatic derivatives of these transfectant cells directly correlated with increased invasiveness in vitro. These studies strongly support the hypothesis that expression of Na+ channel protein and the metastatic behaviour of prostatic carcinoma cells are functionally related, either by endowing the membranes of these cells with specialised electrophysiological properties (e.g. enhancing their motility and/or secretory activities) and/or by perturbing endogenous mechanisms regulating ionic homeostasis within the cells.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020305554ZK.pdf | 142KB | download |