Sodium channel protein expression enhances the invasiveness of rat and human prostate cancer cells

FEBS Letters

Djamgoz, Mustafa B.A²  Ke, Youqiang¹  Foster, Christopher S¹  Fraser, Scott P²  Smith, Paul¹  Rhodes, Nicholas P³  Shortland, Adam P³ 

[1] Department of Cellular and Molecular Pathology, University of Liverpool, Liverpool L69 3GA, UK;Department of Biology, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK;Department of Clinical Engineering, University of Liverpool, Liverpool L69 3GA, UK

关键词: Prostatic carcinoma;    Flow cytometry;    Na+ channel;    Metastasis;    Tissue culture;   

DOI  :  10.1016/S0014-5793(98)00050-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Expression of Na⁺ channel protein was analysed in established cell lines of rat and human prostatic carcinoma origin by flow cytometry using a fluorescein-labelled polyclonal antibody. In many cell lines examined, the obtained frequency distribution profiles were bimodal and identified a subpopulation of cells which expressed high levels of Na⁺ channel protein. A significant positive correlation was demonstrated between the proportion of channel-expressing cells and the functional ability of individual cell lines to invade a basement membrane matrix in vitro. In addition, two transfectant cell lines containing rat prostate cancer genomic DNA were found to express significantly elevated levels of Na⁺ channel protein when compared with the original benign recipient cell line. Enhanced Na⁺ channel expression by two metastatic derivatives of these transfectant cells directly correlated with increased invasiveness in vitro. These studies strongly support the hypothesis that expression of Na⁺ channel protein and the metastatic behaviour of prostatic carcinoma cells are functionally related, either by endowing the membranes of these cells with specialised electrophysiological properties (e.g. enhancing their motility and/or secretory activities) and/or by perturbing endogenous mechanisms regulating ionic homeostasis within the cells.

【 授权许可】

Unknown