Thrombopoietin stimulates VEGF release from c‐Mpl‐expressing cell lines and haematopoietic progenitors

FEBS Letters

Bobik, Renata¹  Hong, Ying¹  Erusalimsky, Jorge D¹  Breier, Georg²  Martin, John F¹ 

[1] Cruciform Project and Department of Medicine, Rayne Institute, University College London, 5 University Street, London WC1E 6JJ, UK;Department of Molecular Cell Biology, Max-Planck-Institut fur physiologische und klinische Forschung, W.G. Kerckhoff Institut, 61231 Bad Nauheim, Germany

关键词: Thrombopoietin;    Vascular endothelial growth factor;    Megakaryocyte;   

DOI  :  10.1016/S0014-5793(98)00056-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Vascular endothelial growth factor (VEGF) production was analysed in megakaryocytic cell lines and CD34⁺ haematopoietic progenitors following treatment with thrombopoietin (TPO). In CMK cells TPO caused a time- and dose-dependent increase in the levels of VEGF released into the medium. A similar effect was observed in UT-7/mpl cells transfected with the TPO receptor c-Mpl, but not in parental UT-7 cells. In CD34⁺ haematopoietic progenitor cell cultures TPO stimulated VEGF mRNA expression and VEGF protein release. Production of VEGF in CD34⁺ cultures increased with TPO-induced megakaryocytic differentiation, but not with erythroid or myelomonocytic differentiation induced respectively by erythropoietin and granulocyte-macrophage colony-stimulating factor. These results demonstrate that TPO stimulates VEGF release in c-Mpl-expressing cells and suggest that this process is an integral feature of the megakaryocytic differentiation programme.

【 授权许可】

Unknown