Vascular types I and II transforming growth factor‐beta receptor expression: differential dependency on tyrosine kinases during induction by TGF‐β

FEBS Letters

Jennings, Garry¹  Agrotis, Alex¹  Ward, Michael R¹  Bobik, Alex¹ 

[1] Cell Biology Laboratory, Baker Medical Research Institute and Alfred Hospital, Prahran, Vic., Australia

关键词: Transforming growth factor-β;    Tyrosine kinase inhibition;    Transforming growth factor-β receptor gene expression;    Vascular smooth muscle;   

DOI  :  10.1016/S0014-5793(98)00011-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recent evidence indicates that the type II transforming growth factor-β (TGF-β) receptor (TβRII) is a serine-threonine-tyrosine kinase. However, the significance of its tyrosine kinase is unclear. We investigated in vascular smooth muscle cells the effects of tyrosine kinase inhibition on the expression of TGF-β receptor types I (ALK-5) and II (TβRII) mRNA, induced by TGF-β ₁. TGF-β ₁ elevated ALK-5 mRNA levels 5-fold; essentially similar TGF-β ₁-dependent elevations were observed with growth factors, PDGF-BB and FGF-2. The tyrosine kinase inhibitor genistein abolished these TGF-β ₁ and growth factor responses. TGF-β ₁ also elevated TβRII mRNA levels which were not inhibited by genistein. We conclude that tyrosine kinases participate in defining how cells respond to TGF-β.

【 授权许可】

Unknown