The 5′ untranslated region of cucumber mosaic virus RNA4 confers highly competitive activity on heterologous luciferase mRNA in cell‐free translation

FEBS Letters

Paek, Kyung Hee¹  Chung, Won-Il²  Kwon, Chang Seob² 

[1] Department of Agricultural Biology, College of Natural Resources and Graduate School of Biotechnology, Korea University, Seoul, 136-701, South Korea;Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon 305-701, South Korea

关键词: Cucumber mosaic virus;    Competitive determinant;    5′ Untranslated region;    mRNA competition;    Translation efficiency;    CMV;    cucumber mosaic virus;    UTR;    untranslated region;    BMV;    brome mosaic virus;    AMV;    alfalfa mosaic virus;    PCR;    polymerase chain reaction;    CP;    coat protein;    ORF;    open reading frame;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/S0014-5793(97)01599-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cell-free translation of virion RNAs of several tripartite RNA viruses has shown that RNA4, a subgenomic RNA, is more competitive than other virion RNAs. Recently, the 3′ untranslated region (UTR) of alfalfa mosaic virus (AMV) RNA4 was identified to be a competitive determinant. In this study, we observed that the RNA4 of cucumber mosaic virus (CMV), another tripartite RNA virus, was also found to be a strong competitor in translational competition among CMV virion RNAs. To identify the competitive determinant of CMV RNA4, we constructed various chimeric luciferase mRNAs containing RNA4 and/or vector-derived UTRs. The relative translations of luciferase-containing mRNA in the presence of a competitor mRNA showed that the 5′ UTR, not the 3′ UTR, substantially contributed to the highly competitive activity of CMV RNA4.

【 授权许可】

Unknown