Study of complexes of a tryptophan‐free mutant of E. coli trp aporepressor with tryptophan analogues using optically detected magnetic resonance (ODMR)

FEBS Letters

Ozarowski, Andrzej¹  Maki, August H¹  Wu, Jie Qiang¹ 

[1] Department of Chemistry, University of California, Davis, CA 95616, USA

关键词: Phosphorescence;    Ootically defined magnetic resonance spectroscopy;    Escherichia coli trp repressor;    Tryptophan analogue;    4-;    5-;    6-FW;    4-;    5-;    and 6-fluorotryptophan;    4-;    5-;    and 6-MeW;    4-;    5-;    and 6- methyltryptophan;    5-BrW;    5-bromotryptophan;    5-HW;    5-hydroxytryptophan;    7-AW;    7-azatryptophan;    apo-trpR;    wild type trp aporepressor from Escherichia coli;    EEDOR;    electron-electron double resonance;    EG;    ethylene glycol;    HAE;    heavy atom effect;    MIDP;    microwave-induced delayed phosphorescence;    ODMR;    optical detection of magnetic resonance;    SLR;    spin-lattice relaxation;    Ti (i=x;    y or z);    a triplet state sublevel;    trpR;    wild type trp holorepressor from Escherichia coli;    W19;    99F;    tryptophan-free mutant of apo-trpR;    zfs;    zero-field splittings;   

DOI  :  10.1016/S0014-5793(97)01602-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphorescence and optically detected magnetic resonance (ODMR) spectra of tryptophan (W) and several of its analogues (4-, 5-, 6-methyltryptophan (MeW); 4-, 5-, 6-fluorotryptophan (FW); 5-bromotryptophan) are compared with those of complexes formed with the W-free trp aporepressor from Escherichia coli (W19,99F). W19,99F binds W and each analogue except 4-FW with an estimated K _D≤30 μM; triplet state spectroscopic and kinetic effects that accompany binding at the corepressor site are reported. ODMR data for the MeW isomers are presented for the first time. No binding of 7-azaW is observed, in agreement with the low affinity found by previous workers.

【 授权许可】

Unknown