Hydrogen peroxide is not released following reaction of cyanide with several catalytically important derivatives of cytochrome c oxidase

FEBS Letters

Palmer, Graham¹  Fabian, Marian¹ 

[1] Department of Biochemistry and Cell Biology MS 140, Rice University, 6100 Main, Houston, TX 77005-1892, USA

关键词: Cytochrome c oxidase;    Peroxy derivative;    Oxyferryl derivative;    Hydrogen peroxide;    Cyanide;    CcO;    cytochrome c oxidase;    CcO-O;    oxidized cytochrome c oxidase;    CcO·CN;    cytochrome c oxidase-cyanide derivative;    P (CcO-607);    peroxy form of cytochrome c oxidase;    F (CcO-580);    oxyferryl form of cytochrome c oxidase;    HRP;    horse radish peroxidase;    MES;    4-morpholine ethane-sulfonic acid;    DM;    N-dodecyl-β-d-maltoside;    TX-100;    Triton X-100;    Tris;    Tris hydroxymethylaminomethane;   

DOI  :  10.1016/S0014-5793(97)01561-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have looked for the production of hydrogen peroxide following reaction of oxidized cytochrome c oxidase and two oxy derivatives (compounds P and F) with cyanide. In each case the final product was the cyanide adduct of cytochrome c oxidase. In no case release of hydrogen peroxide was detected, as gauged by the scopoletin plus horse radish peroxidase assay. The simplest conclusion is that none of these forms of the enzyme contains intact hydrogen peroxide.

【 授权许可】

Unknown