The carboxyl‐terminal domain of insulin‐like growth factor‐I receptor interacts with the insulin receptor and activates its protein tyrosine kinase

FEBS Letters

Zick, Yehiel²  Koval, Anatolii⁴  Hayward, Amanda³  Termini, John¹  Fujita-Yamaguchi, Yoko¹  LeRoith, Derek⁴  Li, Shu-lian¹  Siddle, Ken³ 

[1]Department of Molecular Biology, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, CA, 91010, USA

[2]Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel

[3]Department of Clinical Biochemistry, University of Cambridge, Cambridge, UK

[4]Diabetes Branch, NIDDK, NIH, Bethesda, MD 20892, USA

关键词: Insulin-like growth factor-I receptor;    Insulin receptor;    Protein tyrosine kinase;    Carboxyl-terminal domain;    BIAcore;    Domain interaction;    IR;    insulin receptor;    IGF;    insulin-like growth factor;    PTK;    protein tyrosine kinase;    CT;    carboxyl-terminal;    PDH;    pyruvate dehydrogenase;    MAP kinase;    mitogen-activated protein kinase;    PI;    phosphatidylinositol;    GST;    glutathione S-transferase;    IPTG;    isopropylthio-β-d-galactoside;    CHAPS;    3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate;    RU;    resonance unit;    NTA;    N-(5-amino-1-carboxypentyl) iminodiacetic acid;    NHS;    N-hydroxysuccinimide;    EDC;    N-ethyl-N′-(3-diethylaminopropyl)-carbodiimide;    PDEA;    2-(2-pyridinyldithio)ethaneamine hydrochloride;    DMF;    N;    N-dimethyl formamide;   

DOI  :  10.1016/S0014-5793(97)01523-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Receptors for insulin and insulin-like growth factor-I (IR and IGFIR) consisting of the α₂β₂ structure are protein tyrosine kinases (PTKs). Carboxyl-terminal (CT) domains of their β subunits are structurally diverse while the PTK domains share the highest homology. Interactions between CT and PTK domains of IR and IGFIR were studied by means of PTK activity, fluorescence energy transfer or surface plasmon resonance using BIAcore. We present evidence that IGFIR CT directly interacts with both IGFIR and IR. Although binding to both receptors, stimulation of PTK activity only occurs with IR but not IGFIR.

【 授权许可】

Unknown