Functional expression of pharaonis phoborhodopsin in Eschericha coli

FEBS Letters

Shimono, Kazumi¹  Iwamoto, Masayuki¹  Kamo, Naoki¹  Sumi, Masato¹ 

[1] Laboratory of Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060, Japan

关键词: pharaonis phoborhodopsin (ppR);    pharaonis sensory rhodopsin II (psRII);    Eubacterial expression;    Photocycle;    Flash-photolysis;    ppR;    pharaonis phoborhodopsin;    IPTG;    isopropyl-1-thio-β-galactoside;    CHES;    2-cyclohexylamino-ethanesulfonic acid;   

DOI  :  10.1016/S0014-5793(97)01487-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pharaonis phoborhodopsin, the photoreceptor of the negative phototaxis of archaebacterial Natronobacterium pharaonis, was functionally expressed in the heterologous system of Escherichia coli. Flash-photolysis on a millisecond time scale indicated that the photochemical properties of ppR expressed in E. coli were the same as those of the native ppR in N. pharaonis. We concluded that the integral membrane protein ppR is correctly folded in vivo in the eubacterial E. coli membrane.

【 授权许可】

Unknown