Maturation and degradation of β‐galactosidase in the post‐Golgi compartment are regulated by cathepsin B and a non‐cysteine protease

FEBS Letters

Callahan, John W²  Murata, Mitsuo³  Suzuki, Yoshiyuki¹  Zhang, Sunqu¹  Okamura-Oho, Yuko¹  Oshima, Akihiro¹ 

[1] Department of Clinical Genetics, The Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo 113, Japan;Division of Neurosciences, Research Institute, Hospital for Sick Children, Toronto, Ont. M5G 1X8, Canada;Research Center, Taisho Pharmaceutical Co., Omiya, Saitama 330, Japan

关键词: β-Galactosidase;    Lysosome;    Protease inhibitor;    Cathepsin B;    E-64c;    CA074;    CA074;    N-(1;    3-transpropylcarbamoyloxirane-2-carbonyl)-1-isoleucyl-1-proline;    CA074Me;    N-(1;    3-transpropylcarbamoyloxirane-2-carbonyl)-1-isoleucyl-1-proline methyl ester;    E64-c;    l-3-carboxy-2;    3-trans-epoxypropionyl-leucylamide-(3-methyl)butane;    E64-d;    {N-[-(l-3-trans-ethoxycarbonyloxirane-2-carbonyl-l-leucyl]-3-methyl-butylamine};    4MU;    4-methylumbelliferone;    Z-Arg-Arg-AMC;    carboxybenzoyl-l-argininyl-l-arginine 4-methyl-coumaryl-7-amide;    CHO;    Chinese hamster ovary;    M6P;    mannose 6-phosphate;    MPR;    mannose 6-phosphate receptor;    DMSO;    dimethyl sulfoxide;   

DOI  :  10.1016/S0014-5793(97)01461-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Lysosomal β-galactosidase precursor is processed to a mature form and associated with protective protein in lysosomes. In this study we used two cysteine protease proinhibitors, E64-d for cathepsins B, S, H, and L, and CA074Me for cathepsin B. They are converted intracellularly to active forms, E-64c and CA074, respectively. Both active compounds inhibited maturation of the exogenous β-galactosidase precursor, but E-64c did not inhibit further degradation to an inactive 50-kDa product. We concluded that cathepsin B participated exclusively in maturation of β-galactosidase, and a non-cysteine protease was involved in further degradation and inactivation of the enzyme molecule.

【 授权许可】

Unknown