Biotin synthesis in higher plants: purification and characterization of bioB gene product equivalent from Arabidopsis thaliana overexpressed in Escherichia coli and its subcellular localization in pea leaf cells

FEBS Letters

Alban, Claude¹  Baldet, Pierre²  Douce, Roland¹ 

[1] Unité Mixte CNRS/Rhône-Poulenc (UM 41), Rhône-Poulenc Agrochimie, 14-20 rue Pierre Baizet, 69263 Lyon Cedex 09, France;INRA, Unité de Physiologie Végétale, BP 81, 33883 Villenave d'Ornon Cedex, France

关键词: Biotin biosynthesis;    Biotin synthase;    Arabidopsis thaliana;    Intracellular compartmentation;    DTT;    1;    4-dithiothreitol;    PMSF;    phenylmethylsulfonyl fluoride;    PVP-25;    polyvinylpyrrolidone (M r 25 000);    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    EDTA;    ethylenediaminetetraacetic acid;   

DOI  :  10.1016/S0014-5793(97)01458-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Biotin synthase catalyses the final step in the biotin biosynthetic pathway and is encoded by the bioB gene in Escherichia coli. To investigate the conversion of dethiobiotin to biotin in the plant kingdom, the cDNA encoding the bioB gene product equivalent from Arabidopsis thaliana was used to construct an E. coli overexpression strain. The purified A. thaliana bioB gene product is a homodimer (100 kDa) with a reddish color and has an absorbance spectrum characteristic of protein with [2Fe-2S] clusters. Its intracellular compartmentation in pea leaves discloses a unique polypeptide of 39 kDa within the matrix of mitochondria.

【 授权许可】

Unknown