期刊论文详细信息
FEBS Letters | |
Isolation by differential display and characterization of a tobacco auxin‐responsive cDNA Nt‐gh3, related to GH3 | |
Roux, Camille1  Perrot-Rechenmann, Catherine1  | |
[1] Institut des Sciences Végétales, UPR 0040, CNRS, Bât. 23, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France | |
关键词: Differential display; Early auxin-responsive gene; GH3; Gene expression; Auxin; Tobacco; | |
DOI : 10.1016/S0014-5793(97)01447-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
By use of differential display, we have isolated a new early auxin-responsive cDNA in Nicotiana tabacum. Nt-gh3 had 70% identity with the unique GH3 sequence isolated in soybean by Hagen et al. [Planta 162 (1984) 147–153] and is thus the first reported cDNA related to this gene until now. Nt-gh3 mRNA accumulates within a short time after auxin treatment, responds to very low concentrations of NAA (as little as 10−9 M) and specifically to active auxins. Nt-gh3 mRNA is demonstrated to be one of the most relevant early molecular markers of primary auxin response.
【 授权许可】
Unknown
【 预 览 】
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