Isolation by differential display and characterization of a tobacco auxin‐responsive cDNA Nt‐gh3, related to GH3

FEBS Letters

Roux, Camille¹  Perrot-Rechenmann, Catherine¹ 

[1] Institut des Sciences Végétales, UPR 0040, CNRS, Bât. 23, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France

关键词: Differential display;    Early auxin-responsive gene;    GH3;    Gene expression;    Auxin;    Tobacco;   

DOI  :  10.1016/S0014-5793(97)01447-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By use of differential display, we have isolated a new early auxin-responsive cDNA in Nicotiana tabacum. Nt-gh3 had 70% identity with the unique GH3 sequence isolated in soybean by Hagen et al. [Planta 162 (1984) 147–153] and is thus the first reported cDNA related to this gene until now. Nt-gh3 mRNA accumulates within a short time after auxin treatment, responds to very low concentrations of NAA (as little as 10⁻⁹ M) and specifically to active auxins. Nt-gh3 mRNA is demonstrated to be one of the most relevant early molecular markers of primary auxin response.

【 授权许可】

Unknown