Micro‐injection of recombinant lysyl oxidase blocks oncogenic p21‐Ha‐Ras and progesterone effects on Xenopus laevis oocyte maturation

FEBS Letters

Giampuzzi, Monia¹  Lacal, Juan Carlos²  Di Duca, Marco¹  Ghiggeri, Gianmarco¹  Di Donato, Armando¹  Gusmano, Rosanna¹ 

[1] Department of Nephrology, Institute G. Gaslini, Largo G. Gaslini, 5, 16147 Genoa, Italy;Instituto de Investigaciones Biomedicas, CSIC, Calle Arturo Duperier, 4, 28029 Madrid, Spain

关键词: Lysyl oxidase;    Cross-link;    Ras;    Progesterone;    Germinal vesicle breakdown;    Xenopus laevis;   

DOI  :  10.1016/S0014-5793(97)01420-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previous evidence suggested an anti-oncogenic role for lysyl oxidase, mainly in ras-transformed cells. Here we prove that recombinant lysyl oxidase is actually able to antagonize p21-Ha-Ras-induced Xenopus laevis oocyte maturation. Lysyl oxidase was also effective on progesterone-dependent maturation, indicating a block lying downstream of Ras. Maturation induced by activated ‘maturation promoting factor’, normally triggered by progesterone, was also inhibited by lysyl oxidase. Finally, lysyl oxidase did not abolish p42^Erk2 phosphorylation upon maturation triggering, suggesting a block downstream of Erk2. Further investigation showed that lysyl oxidase action depends on protein synthesis and is therefore probably mediated by a newly synthesized protein.

【 授权许可】

Unknown