Cloning and transcriptional regulation of the gene encoding the vacuolar/H+ ATPase B subunit of Dictyostelium discoideum

FEBS Letters

Peracino, Barbara¹  Noegel, Angelika A²  Bracco, Enrico¹  Bozzaro, Salvatore¹ 

[1] Dipartimento di Scienze Cliniche e Biologiche, Università di Torino, Ospedale S. Luigi Gonzaga, 10043 Orbassano-Turin, Italy;Max-Planck-Institut für Biochemie, Am Klopferspitz 18a, 82152 Martinsried, Germany

关键词: V/H+ ATPase;    Transcriptional regulation;    Phagocytosis;    Endocytosis;    Dictyostelium;   

DOI  :  10.1016/S0014-5793(97)01425-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The main function of vacuolar H⁺ ATPases in eukaryotic cells is to generate proton and electrochemical gradients across the membrane of inner compartments. We have isolated the gene encoding the B subunit of Dictyostelium discoideum vacuolar H⁺ ATPase (vatB) and analyzed its transcriptional regulation. The deduced protein comprises 493 amino acids with a calculated molecular mass of 54 874 Da. The predicted protein sequence is highly homologous to previously determined V/H⁺ ATPase B subunit sequences. The protein is encoded by a single gene in the Dictyostelium genome. The gene is maximally expressed during growth and it decreases during the first hours of development. Gene expression is rapidly enhanced by phagocytosis, but not by fluid-phase endocytosis. Acidic and alkaline conditions affect vatB gene expression differently.

【 授权许可】

Unknown