Primary structure and expression of a naturally truncated human P2X ATP receptor subunit from brain and immune system

FEBS Letters

Babinski, Kazimierz¹  Nouel, Dominique¹  Paquet, Michel¹  Séguéla, Philippe¹  Lê, Khanh-Tuoc¹ 

[1] Cell Biology of Excitable Tissue Group, Montreal Neurological Institute, McGill University, 3801 University, Montreal, Que. H3A 2B4, Canada

关键词: Transmitter-gated channel;    Purinoceptor;    Nucleotide;    Lymphocyte;    Cerebellum;   

DOI  :  10.1016/S0014-5793(97)01380-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel member of the ionotropic ATP receptor gene family has been identified in human brain. This 422 amino acid long P2X receptor subunit has 62% sequence identity with rat P2X₅. Several characteristic motifs of ATP-gated channels are present in its primary structure, but this P2X₅-related subunit displays a single transmembrane domain. Heterologous expression of chimeric subunits containing the C-terminal domain of rat P2X₅ leads to the formation of desensitizing functional ATP-gated channels in Xenopus oocytes. The developmentally regulated mRNA, found in two splicing variant forms, is expressed at high levels in brain and immune system.

【 授权许可】

Unknown