Ribosomal efficiency and growth rates of freshly isolated Escherichia coli strains originating from the gastrointestinal tract

FEBS Letters

Mikkola, Riitta⁴  Conway, Patricia L³  Molin, Sören¹  Rang, Camilla U² 

[1] Department of Microbiology, The Technical University of Denmark, Building 301, 2800 Lyngby, Denmark;Department of General and Marine Microbiology, Göteborg University, Medicinaregatan 9C, 413 90 Göteborg, Sweden;CRC for Food Industry Innovation, School of Microbiology and Immunology, University of New South Wales, Sydney, NSW 2052, Australia;Department of Molecular Biology, Uppsala University, Biomedical Center, Box 590, 751 24 Uppsala, Sweden

关键词: Enteric isolate;    Escherichia coli;    Ribosomal efficiency;    Growth rate;   

DOI  :  10.1016/S0014-5793(97)01341-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It has been previously reported that for natural Escherichia coli isolates from the ECOR collection, there were differences in the ribosomal efficiencies and there was a direct correlation between growth rate and the ribosome efficiency (R-factor). The aim of this study was to determine whether strains freshly isolated (i.e. subcultured <5 times) from the gastrointestinal tract ecosystem also exhibited this correlation. Eleven E. coli and two Enterobacter spp. isolates from either humans, pigs, rats or a mammoth were investigated. Considerable variability in the R-factor was noted using an in vitro translation assay, however no consistent correlation between the R-factor and growth rate was noted.

【 授权许可】

Unknown