Kidney‐specific expression of a novel mouse organic cation transporter‐like protein

FEBS Letters

Mukoyama, Masashi¹  Tashiro, Kei²  Aoki, Tomohiro¹  Kuwahara, Takashi³  Ogawa, Yoshihiro¹  Ozaki, Shoichi¹  Ebihara, Ken¹  Tanaka, Issei¹  Nakao, Kazuwa¹  Sugawara, Akira³  Tamura, Naohisa¹  Mori, Kiyoshi¹ 

[1]Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto 606, Japan

[2]Center for Molecular Biology and Genetics, Kyoto University, Kyoto 606, Japan

[3]Department of Nephrology, Saiseikai Nakatsu Hospital, Osaka 530, Japan

关键词: Organic cation transporter;    Nutrient transporter;    Signal sequence trap;    In situ hybridization;    Mouse kidney;    cDNA;    complementary DNA;    cRNA;    complementary RNA;    RACE;    rapid amplification of cDNA ends;   

DOI  :  10.1016/S0014-5793(97)01325-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using the signal sequence trap method, we have cloned a novel 12-membrane-spanning transporter-like protein, termed renal-specific transporter (RST), from the mouse kidney. RST is a 553-amino-acid protein highly homologous to recently cloned organic cation transporters, e.g. it is 30% identical to rat organic cation transporter 1 at the amino acid level. Northern blot analysis has revealed that the RST gene is expressed abundantly and specifically in the kidney. In situ hybridization analysis has shown that RST gene expression is restricted to the renal proximal tubule, where various organic cations such as endogenous catecholamines and choline or clinically used cationic drugs are known to be actively excreted.

【 授权许可】

Unknown