Glycosylation of RNA polymerase II from wheat germ

FEBS Letters

Eufemi, Margherita¹  Ciavatta, Patrizia¹  Marmocchi, Franco²  Ferraro, Anna¹  Turano, Carlo¹  Cervoni, Laura¹ 

[1] Department of Biochemical Sciences ‘A. Rossi Fanelli' and CNR Center of Molecular Biology, University ‘La Sapienza', P.le Aldo Moro 5, Rome, Italy;Department of Biology M.C.A., University of Camerino, Camerino, Italy

关键词: RNA polymerase II;    Calf thymus;    Wheat germ;    Glycosylation;   

DOI  :  10.1016/S0014-5793(97)01285-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

RNA polymerase II from wheat germ was analyzed for the presence of sugars. The two largest subunits and the 27 and 25 kDa subunits were found to be glycosylated by a variety of sugars. However, no N-acetylglucosamine was detected, which was found by Kelly et al. (J. Biol. Chem. (1993) 268, 10416–10424) in the largest subunit of RNA polymerase II from calf thymus. Thus it appears that the regulatory function of this sugar, postulated by Kelly et al., is performed in the wheat germ enzyme by other monosaccharides. Carbohydrate analysis of the two largest subunits of the calf thymus enzyme also revealed the presence, beside N-acetylglucosamine, of other sugars. Some similarities in the features of glycosylation of the two polymerases, isolated from very different organisms, suggest that the sugar moieties have an important role in the structure and/or function of these enzymes.

【 授权许可】

Unknown