FEBS Letters | |
Early expression of a β1‐adrenergic receptor and catecholamines in Xenopus oocytes and embryos | |
Paquereau, L.2  Devic, E.2  Caput, D.1  Steinberg, R.3  Audigier, Y.2  | |
[1] Sanofi Recherches, Centre de Labège, Voie No. 1, B.P. 137, Labège Innopole, 31676 Labège Cedex, France;Unité U-397 INSERM, CHU Rangueil, Bât. L3, 1 Avenue Jean-Poulhès, 31405 Toulouse Cedex 4, France;Sanofi Recherches, 371 rue du Professeur J. Blayac, Montpellier Cedex 04, France | |
关键词: Early embryogenesis; Xenopus; β-Adrenergic receptor; Catecholamine; | |
DOI : 10.1016/S0014-5793(97)01278-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
From a Xenopus stage 11 cDNA library, we have cloned a gene, termed X-β 1AR, whose sequence is highly homologous to that of the human β1-adrenergic receptor. As shown by RT-PCR assay, X-β 1AR RNA is present in the mature oocyte, decreases after fertilization up to stage 6 and then gradually increases during gastrulation. Binding studies performed with radiolabeled ligands reveal that X-β 1AR RNA is translated into the receptor protein. Furthermore, noradrenaline and adrenaline are also detected in oocytes and early embryos. The concomitant presence of β1-adrenergic receptors and catecholamines suggest that this ligand-receptor couple could play a role in the very early stages of embryonic development.
【 授权许可】
Unknown
【 预 览 】
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