Early expression of a β1‐adrenergic receptor and catecholamines in Xenopus oocytes and embryos

FEBS Letters

Paquereau, L.²  Devic, E.²  Caput, D.¹  Steinberg, R.³  Audigier, Y.² 

[1] Sanofi Recherches, Centre de Labège, Voie No. 1, B.P. 137, Labège Innopole, 31676 Labège Cedex, France;Unité U-397 INSERM, CHU Rangueil, Bât. L3, 1 Avenue Jean-Poulhès, 31405 Toulouse Cedex 4, France;Sanofi Recherches, 371 rue du Professeur J. Blayac, Montpellier Cedex 04, France

关键词: Early embryogenesis;    Xenopus;    β-Adrenergic receptor;    Catecholamine;   

DOI  :  10.1016/S0014-5793(97)01278-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

From a Xenopus stage 11 cDNA library, we have cloned a gene, termed X-β 1AR, whose sequence is highly homologous to that of the human β₁-adrenergic receptor. As shown by RT-PCR assay, X-β 1AR RNA is present in the mature oocyte, decreases after fertilization up to stage 6 and then gradually increases during gastrulation. Binding studies performed with radiolabeled ligands reveal that X-β 1AR RNA is translated into the receptor protein. Furthermore, noradrenaline and adrenaline are also detected in oocytes and early embryos. The concomitant presence of β₁-adrenergic receptors and catecholamines suggest that this ligand-receptor couple could play a role in the very early stages of embryonic development.

【 授权许可】

Unknown